Cytotoxic effect of pyocyanin on human pancreatic cancer cell line (Panc-1)
Authors
Abstract:
Objective(s): Pyocyanin, a blue-green pigment produced by Pseudomonas aeruginosa, interferes with host redox cycles, which can lead to generation of reactive oxygen species and progressive cellular oxidative damage. The aim of this study was to assess the influence of pyocyanin on human pancreatic cancer cell line.Materials and Methods: Polymerase Chain Reaction (PCR) was applied to confirm the existence of a specific pyocyanin producing gene (phzM). The pigment was then characterized by UV-visible, FT-IR, and HPLC analysis. Panc-1 cells were treated by different concentrations of pyocyanin and their cytotoxic effect as well as the induction of apoptosis/necrosis were evaluated by XTT assay and flow cytometry.Results: An overnight pyocyanin treatment resulted in significant cell reduction in a concentration-dependent manner. Inhibition rate of 6 mg.ml-1 pyocyanin (the highest concentration) extracted from clinical and soil isolates of P. aeruginosa were 98.69±0.23 and 89.88±1.86%, respectively, which decreased as the pyocyanin concentration lessened. Pyocyanin could also induce dose-dependent apoptosis/necrosis in Panc-1 cells after 24 hr.Conclusion: We reported, for the first time, cytotoxic effects of pyocyanin against human pancreatic cancer cell line. Considering this effect of the pigment, study on pyocyanin as a potential anti-tumor biodrug requires further studies.
similar resources
effects of disulfiram on apoptosis in panc-1 human pancreatic cancer cell line
pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. it is characterized by a poor diagnosis and lack of response to conventional therapy. recent studies have suggested that disulfiram (dsf), a member of the dithiocarbamate family, may have antitumor activity. this study aimed to evaluate the in vitro eff...
full textEffects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the dithiocarbamate family, may have antitumor activity. This study aimed to evaluate the in vitro eff...
full textMetabolic characterization of invaded cells of the pancreatic cancer cell line, PANC‐1
We previously reported that about 0.4% of cells in the cultured human pancreatic cancer cell line, PANC-1, can invade matrigel during the transwell invasion assay, suggesting that these invaded PANC-1 cells may have specific characteristics to keep their invasive potential. To identify the metabolic characterization specific in the invaded PANC-1 cells, metabolome analysis of the invaded PANC-1...
full textWild Type p53 Gene Transfer Increases Chemosensitivity and Apoptotic Response of PANC-1 Pancreatic Tumor Cell Line
The effect of p53 gene therapy on chemosensitivity and apoptotic response of PANC-1 tumor cells, which express high amount of mutant p53, to cancer chemotherapeutic agents of Etoposide and Doxorubicin was investigated. Comparison of the chemosensitivity of PANC-1 cells to its wild type p53 transfectants showed that wt-p53 expressing transfectants are more sensitive to both Etoposide and Doxorub...
full textThe Cytotoxic Effect of Hyssop Extract on Breast Cancer Cell Line
Background and Objectives: Given the rising prevalence of breast cancer in Iran over the past two decades and also high mortality rate of the patients, and on the other hand, according to oral administration, low cost, and easy public access to hyssop (Hyssopus officinalis), in this study, the effectiveness of this herbal product, was investigated on MCF-7 breast cancer cell line. Methods: I...
full textCytotoxic Effect of Capparis spinosa L. on the PLC/PRF/5 Human Hepatocellular Carcinoma Cell Line
ABSTRACT Background and Objectives: Capparis spinosa has been used in traditional medicine for various applications including treatment of liver disorders and cancer. We studied the effects of this plant on cell proliferation and morphological characteristics of PLC/PRF/5 liver cancer cell line. Method...
full textMy Resources
Journal title
volume 21 issue 8
pages 794- 799
publication date 2018-08-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023